The extract was also subjected to acute toxicity test and phytochemical analysis. Rat adjuvant arthritis aia is an experimental model of polyarthritis that has been widely used for preclinical testing of numerous antiarthritic agents, which are either under preclinical or clinical investigation, or are currently used as therapeutics in this disease 24. Vge did not show any toxic symptoms even at a dose of 2000 mgkg in acute toxicity studies on rats. Though, it has not been pharmacologically assessed for rheumatoid arthritis. Majoon suranjan ms is a polyherbal formulation used in unani system of medicine for the treatment of rheumatoid arthritis ra. Wistar rats with formaldehydeinduced arthritis were divided into six. Significant antiarthritic activity was observed in subacute and chronic models of formaldehyde. Establishment of a rat adjuvant arthritisinterstitial. Antiinflammatory activity of compounds isolated from plants. This model was expanded to mice 2 and nonhuman primates 3 and has been widely used. Protocol for adjuvantinduced arthritis aia in rats.
Conventional f344 rats develop aia, but arthritis is less severe and incidence is low as 20%. All wistar rats were divided into five groups, group i served as normal control and received vehicle only, group iiiv received different doses 125, 250, 500 mgkg, p. Joint swelling was measured in formaldehyde induced arthritis. Formaldehydeinduced arthritis in rats was carried out according to the method described by gupta et al. The aim of this study was to evaluate the effects of the injection of two phlogistic agents, carrageenan and formalin, in the rat tmj, and the inflammatory process induced by these substances. We investigated whether rats with antigeninduced arthritis aia exhibit peripheral changes in skin innervation remote from the inflamed joint. We assessed the effect of a novel and selective phosphodiesterase 4 pde4 inhibitor, ciclamilast, on chronic inflammation in adjuvantinduced arthritis aia, a rat model of rheumatoid arthritis ra, and acute inflammation in the rat and mouse model of. Medicinal uses and pharmacological activities of cyperus. Hematological and lipid profile evaluation of a hexane. Arthritic assessment and body weight was measured at regular interval till 28 days.
This inhibition is not antagonized by sotalol mj 1999, a beta adrenergic blocking agent. Effect of ethanol extract of an ayurvedic preparation. Oa animals were orally dosed with either d003 100 and 400 mgkg or naproxen nap 3 mgkg, as. Catecholamines, which also inhibit the inflammatory response, are blocked by sotalol in this regard. Formaldehyde used to induce arthritis using reported method with minor modification 11. Ciclamilast ameliorates adjuvantinduced arthritis in a. The spread of pain is usually attributed to mechanisms in the central nervous system. Phytopharmacotherapeutics of cyperus rotundus linn. Female lewis rats 100 gm were injected subcutaneously at the base of the tail with 300 l 5 mgml lyophilized mycobacterium butyricum difco, detroit, mi in sterile mineral oil on day 0. Return to article details antiarthritic activity of achyranthes aspera on formaldehyde induced arthritis in rats download download pdf antiarthritic activity of achyranthes aspera on formaldehyde induced arthritis in rats download download pdf. Antiarthritic activity of achyranthes aspera on formaldehydeinduced arthritis in rats. Research article protective effect of aqueous extract of.
Study of the inflammatory process induced by injection of. Mice with collagen antibodyinduced arthritis caia received two intraperitoneal injections of human bone marrowderived mscs. The assessment made on the 10th day showed that, treatment with cyperus rotundus 500 mgkg significantly reduced the swelling in the injected left hind paw as compared to diclofenac sodium. While effective in female rats in either interval, in male rats, ranitidine 10 and methylprednisolone 1 were effective only in the earlier interval, but not in later, whereas omeprazole 10 was completely not effective. For evaluation of inflammation carrageenan, histamine and serotonininduced paw edema served as acute models and formaldehydeinduced arthritis served as a chronic model in rats.
In the present study, 108 rats were allocated into 18 groups six rats each and the study protocols were divided into four stages. Antiinflammatory activity of telmisartan in rat models of. An overview n singh1, b r pandey1, p verma1, m bhalla1 and m gilca2 1international institute of herbal medicine iihm, gomtinagar, lucknow226 010, uttar pradesh, india 2department of biochemistry, faculty of general medicine. Rats were killed at various times postadjuvant injection, and joints harvested. In addition, doses of 2080 mgkg reduced edema and inflammatory infiltrate in rats.
Chronic immunological cfainduced arthritis in rats a induction of arthritis. Acute nonimmunological formaldehydeinduced arthritis in rats. The therapeutic effect of cortisone on chronic rheumatoid arthritis in man hench et al. Effect of ethanol extract of an ayurvedic preparation pathyadya churna on arthritis in rats madhavi g patel 1, kilambi pundarikakshudu 2 1 department of pharmacognosy, parul institute of pharmacy, vadodara, gujarat, india 2 department of pharmacognosy, l. Arthritis was induced by intradermal injection of formaldehyde 0. Pdf therapeutic effect of linum usitatissimum flaxseed. The mean changes in diameter of paw were noted at regular. The lesions in adjuvant arthritis are somewhat less analogous to those seen in human ra than are the lesions of type ii. It produced inhibition of the exudate volume and the leucocyte migration in rats and vascular permeability in mice. Antiarthritic activity of aqueousmethanolic extract and. Induction of rat adjuvantinduced arthritis aia and tissue sampling. The different doses of the ufd were tested against the turpentine oil to, formaldehyde induced acute arthritis and complete fruends adjuvant cfa induced chronic arthritis in wistar rats. Pdf antirheumatoid activity of aqueous extract of piper longum on. This study involved seven groups of six rats each, namely the normal.
The antiinflammatory activity of harungana madagascariensis hypericaceae leaf was evaluated using formaldehydeinduced acute edema and arthritis respectively, and cotton pellet granuloma in rats. Evaluation of the antiarthritic activity of cinnamomum cassia bark. Effects of different concentrations of formalin on paw. Rats with aia show a highly reproducible pathology with welldefined acute and chronic states. Intraperitoneal infusion of mesenchymal stem cell attenuates. Carvedilol can attenuate histamineinduced paw edema and. The present study was undertaken to assess the activityantiinflammatory potential of linum usitatissimum fixed oil against castor oilinduced diarrhoea, turpentine oilinduced joint oedema, formaldehyde and complete freunds adjuvant cfainduced.
Research article investigation of antiarthritic potential. Results of investigations using animal models of inflammation and arthritis e. Histamineinduced rat paw edema rats of this group were divided into 6 subgroups 8 rats. Formaldehyde was used for the induction of acute arthritis. The antiinflammatory action of nepitrin, a flavonoid. The current study explores antiarthritic activity and phytochemical analysis of aqueousmethanolic extract 30. The extract was administered to the rats by oral gavages in different doses. Protocol for induction of adjuvantinduced arthritis aia. The oil was administered intraperitoneally for 14 days in the case of adjuvantinduced arthritis and 10 days in formaldehydeinduced arthritis. Development of an animal model of rheumatoid arthritis interstitial lung disease raild and improved knowledge of the pathogenesis of raild may facilitate earlier diagnosis and the development of more effective targeted therapies.
Animals were randomly divided into five groups of six animal each n6. To examine the effect of koumine, a gelsemium alkaloid, on two experimental models of rheumatoid arthritis ra, rats with adjuvantinduced arthritis aia and collageninduced arthritis cia were administered koumine 0. The aim of this study was to investigate the effects of d003 on formaldehydeinduced oa in rats. Spraguedawley rats were divided into ve groups of six animals each. Antiarthritic activity of a classical ayurvedic formulation vatari. Effects of d003 on formaldehydeinduced osteoarthritis in. The present study evaluates the antiarthritic effect of spirulina fusiformis on formaldehyde induced rheumatoid arthritis in mice. Histamineinduced rat paw edema rats of this group were divided into 6 subgroups 8 rats each. While the stimulus during the early phase is a direct chemical stimulation of the nociceptors, that during.
Antigeninduced arthritis in rats is associated with. Protective effect of pinitol against inflammatory mediators of. Adult male wistar rats were studied in an adjuvant arthritis aa model induced by the injection of freunds complete adjuvant fca. Pain in arthritis may be experienced in regions outside the affected joint, and hyperalgesia may even be widespread. Western africa network of natural products research. Nepitrin was found to possess significant antiinflammatory activity in the exudative and proliferative phases of inflammation. Antiarthritic activity of whole plant extract of pentatropis capensis on. Antiarthritic activity of whole plant extract of pentatropis capensis. Eabp significantly suppressed the joint swelling when compared with arthritic control between day 4 and day 10. View of antiarthritic activity of achyranthes aspera on. Research article investigation of antiarthritic potential of plumeria alba l. It inhibited granuloma formation in the cotton pellet test. Protocol for the successful induction of collageninduced arthritis cia in rats collageninduced arthritis cia was first developed using wistar outbred, spraguedawley outbred and wistarlewis inbred rats by immunization with type ii collagen 1. In complete freunds adjuvant cfainduced arthritis model, cfa was.
Evaluation of complete freunds adjuvantinduced arthritis. In acute tests ea displayed marked antiinflammatory activity. This action of nepitrin could be due to its antibradykinin and antiangiotensin action. Chemokine receptor expression in rat adjuvantinduced. To determine the ameliorative potential of aqueous extract of achyranthesasperaaeaa against arthritis using swiss albino mice and wistar rats, and its possible mechanism of action. Herbal medicinal products in the treatment of osteoarthritis. Indian journal of natural products and resources vol. The administration of curcumin also inhibits formaldehydeinduced arthritis in rats at a dose of 40 mgkg, and this dose has negligible levels on peptic ulcer formation compared to phenylbutazone, and without any acute toxicity at doses of up to 2 gkg 27. The leaf extract was obtained by cold maceration in methanol. Pronounced inflammation of the affected knee joint with local swelling and. The rat formalin test, which causes a local tissue injury of the paw, has been used as a model for tonic pain 1 and localized inflammatory pain 2, 3.
Jordan journal of biological sciences, vol 7, issue 2, 2014, 1118. Swiss albino mice 2530 g and wistar rats 150180 g under standard controlled conditions 24 2c, 5070 humidity and 12 h lightdark cycle. Curcumin also inhibited formaldehydeinduced arthritis in rats at a dose of 40 mgkg, had a lower ulcerogenic index 0. In this study, a total of 45 adult rats were distributed in two experimental groups and a control group. Effect of ms on formaldehyde induced arthritis in rats. In practice, mineral oil, squalane, and liquid paraf.
Thereafter the majority of workersused experimental p. One hour later, arthritis was induced by injecting 0. Hepatoprotective activity of andrographis paniculata in ethanol induced. Effects of koumine on adjuvant and collageninduced. Therapeutic efficacy of spirulina in the treatment of. Antiinflammatory activity of the antihistamines does not correlate well with antihistaminic. Thus, vatari guggulu, a classical ayurvedic formulation of the indian system of medicine, exhibited significant antiarthritic activity in formaldehyde and cfa induced arthritis in rats. Antihistamines, in general, inhibit the formaldehydeinduced inflammatory response in the hind paws of rats. Antinociceptive and antiinflammatory properties of daucus. Results the cfainduced model rats developed redness and nodules in the tail and front paws, and the arthritic control ac group rats showed similar.
Protocol for the successful induction of collageninduced. Longterm effects of epigallocatechin gallate egcg on. Perez g antiinflammatory activity of compounds isolated from plants thescientificworld 2001 1, 7784 718 17 glaucine found in a wide variety of genera in the annonaceae alphonsea, annona, polyalthia, and uvaria. Nonpolar hydrogen atoms were merged, and rotatable bonds were defined. Antiinflammatory effects of an ethyl acetate extract of.
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